Journal: Pflugers Archiv : European journal of physiology

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels.

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Fig. 3 Phosphatropic hormones after 5 days of low- or high- phosphate (Pi) diet. Levels of A serum parathyroid hor- mone (PTH), B plasma intact fibroblast growth factor 23 (iFGF23), C plasma C-terminal FGF23 (cFGF23), D the ratio of iFGF23 (ng/l)/cFGF23 (ng/l) as well as serum E calcidiol, and F calcitriol, and G plasma soluble Klotho, and H Fetuin-A on the final day of both the 5-day low and high Pi diet. One reference unit (RU)/ml corre- sponds to 2 ng/l cFGF23. Blue dots represent differences (Δ) between high Pi diet and low Pi diet expressed as mean differ- ence ± 95% confidence interval and blue dashed line represents the zero line of the right y-axis. Data was analyzed by the paired t-test (A–E) or Wilcoxon test (F–H), n = 10, *p < 0.05

Article Snippet: Daily fractional excretion of Pi (FEPi) was calculated by the following equation using the absolute 24-h urine Pi and creatinine: Daily renal filtered Pi load was calculated with the following equation: Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: The FASEB Journal

Article Title: Lysophosphatidic Acid Synergizes With 1,25‐Dihydroxyvitamin D to Promote Fibroblast Growth Factor‐23 Synthesis via MAPK Signaling and Induction of the IL12A Gene

doi: 10.1096/fj.202502235R

Figure Lengend Snippet: Stimulation of FGF23 production by LPA and 1,25D and the whole transcriptome analysis of Ocy454 cells. (A) Secreted total FGF23 (cFGF23) concentrations in the culture medium of mouse long bone explants in response to vehicle (VEH), LPA alone, 1,25D alone, or LPA/1,25D after 24 h of stimulation. (B) Serum intact FGF23 (iFGF23) levels following the intraperitoneal LPA injection into the wild‐type and VDR KO littermates. (C) Experiment design for whole transcriptome analysis in Ocy454 cells. (D) Ven diagrams of differentially expressed genes (DEGs) in response to LPA, 1,25D, or LPA/1,25D treatment 2 and 8 h after stimulation. (E) Volcano plots of LPA and 1,25D responsive genes demonstrating LPA and 1,25D act on Ocy454 cells. (F) Expression profile of osteocyte genes in response to LPA, 1,25D, or the LPA/1,25D treatment after 2‐ and 8‐h stimulation. One‐way ANOVA followed by Tukey's (A) or two‐way ANOVA followed by Bonferroni (B) post hoc tests for multiple comparisons. Mean ± SEM, n = 3–4 experiments and n = 7–10 mice/group, respectively. DEGs and adjusted p values obtained by Benjamini‐Hochberg (BH) method using Limma package on R studio (D–F). Blue: 1,25D and Red: LPA responsive genes (E). Asterisks represent * p < 0.05, ** p < 0.01, *** p < 0.001 compared to vehicle‐treated group (F).

Article Snippet: After 24‐h incubation, supernatants were collected, cFGF23 concentrations were measured using a cFGF23 ELISA kit (60‐6300, Quidel) according to the manufacturer's instructions, and the cFGF23 concentrations were normalized by bone weight in the corresponding well.

Techniques: Injection, Expressing

Journal: Nephrology (Carlton, Vic.)

Article Title: Oral iron supplementation with sodium ferrous citrate reduces the serum intact and c‐terminal fibroblast growth factor 23 levels of maintenance haemodialysis patients

doi: 10.1111/nep.12909

Figure Lengend Snippet: Subjects’ serum intact FGF23 and C‐terminal FGF23 levels and intact FGF23:C‐terminal FGF23 ratios at the baseline and after 3 months’ sodium ferrous citrate treatment. The iFGF23 (pg/mL) and cFGF23 (pmol/L) measurements were obtained simultaneously, and the unit conversion of the cFGF23 data was performed using the following equation: 0.133 pmol/L = 1 pg/mL. The data are expressed as median values and interquartile ranges. * P < 0.05 compared with the baseline values.

Article Snippet: The patients’ serum iFGF23 (pg/mL) levels were measured using a sandwich ELISA kit (Kainos Laboratories, Tokyo, Japan), as were their serum cFGF23 (pmol/L) levels (Biomedica Medizinprodukte GmbH & Co KG, Vienna, Austria).

Techniques: